Cytiva DEAE-Sephacel ™Media 500mL

Workflow of human aorta proteoglycan extraction, isolation by diethylaminoethyl –Sephacel chromatography and analysis LC-MSMS

The activities of calpastatin, μ-calpain and m-calpain can be rapidly assayed following the separation on DEAE-Sephacel chromatography by a 2 stage elution with

deae column chromatography After this neutralization, each preparation of JH, JUL1 and JUL3 was loaded into a DEAE Sephacel anion-exchange column (5× 25 cm, Pharmacia Biotech, Uppsala

deae resin The supernatant then was clarified by centrifugation at 105,000 x g,, for 60 min and loaded onto a x 40-cm column of DEAE-Sephacel at 24 mlh